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Benzamidine Aogarose ( Sepharose ) 4FF Nickel Affinity Chromatography Resin

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Benzamidine Aogarose ( Sepharose ) 4FF Nickel Affinity Chromatography Resin

China Benzamidine Aogarose ( Sepharose ) 4FF Nickel Affinity Chromatography Resin supplier

Large Image :  Benzamidine Aogarose ( Sepharose ) 4FF Nickel Affinity Chromatography Resin

Product Details:

Place of Origin: China
Brand Name: AOGMA
Certification: Yes
Model Number: Benzamidine Aogarose 4FF

Payment & Shipping Terms:

Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 5ml, 25ml, 100ml, 200ml, 500ml, 1L etc.
Delivery Time: 10 working days
Payment Terms: T/T, L/C
Supply Ability: No limitation
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Detailed Product Description
Matrix: Highly Cross-linked 4% Agarose Gel Average Particle Size: ~ 90μm
Recommend Flow Rate: 30 ~ 300cm/h PH Stability: 2 ~ 8 (working); 1 ~ 9 (CIP)
Chemical Stability: Stable In Most Water Soluble Buffer Functional Group: P-aminobenzamidine
Ligand Density: ≥12μmol P-aminobenzamidine/ml Media Binding Capacity: ≥35 Mg Trypsin/ml Media

 【CHARACTERISTICS】

  Ligand's Amide bond couple to 4% cross-linked agrose gel through a 14 carbon atoms spacer arm.
Benzamidine chemical is broad spectrum inhibitor of serine proteases. Consequently, the agrose with the coupling benzamidine can be used to the further purification process of serine proteases such as : trypsin,thrombin, urokinase, kinins release enzyme,former Kallikrein and so on.
Widely used in biochemical research.

  Benzamidine Aogarose 4FF is suitable for enzyme purification, widely used in biochemical research.

 

【SPECIFICATION】

 

Matrix: Highly Cross-linked 4% Agarose Gel Average Particle Size: ~ 90μm
Recommend Flow Rate: 30 ~ 300cm/h PH Stability: 2 ~ 8 (working); 1 ~ 9 (CIP)
Chemical Stability: Stable In Most Water Soluble Buffer Storage Conditions: 4 ~ 8℃; 20% Ethanol, 50mM Acetic Acid, PH4.0
Sterilization: 121℃,autoclaved Sterilization 20min Functional Group: P-aminobenzamidine
Ligand Density: ≥12μmol P-aminobenzamidine/ml Media Binding Capacity: ≥35 mg Trypsin/ml media

 

【APPLICATION CASE】

 

Chromatographic Column : RTU Fast 5ml Benzamidine Aogarose 4FF

Sample : AACT(Alpha chymotrypsin) protein mixture

Binding buffer : 50mM PB, 500mMNaCl pH 7.0

Elution buffer: 0.1M Gly, pH2.7(Inject 200^1 1M Tris-HCl pH9.0 to Eluent collection tube advance)

Flow rate : 0.7ml/min

 

 

Benzamidine Aogarose ( Sepharose ) 4FF Nickel Affinity Chromatography Resin

 

Contact Details
YELI BIOTECH (SHANGHAI) CO., LTD

Contact Person: emily

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