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Affinity Chromatography media IgM capture Aogarose ( Sepharose ) HP

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Affinity Chromatography media IgM capture Aogarose ( Sepharose ) HP

China Affinity Chromatography media IgM capture Aogarose ( Sepharose ) HP supplier

Large Image :  Affinity Chromatography media IgM capture Aogarose ( Sepharose ) HP

Product Details:

Place of Origin: China
Brand Name: AOGMA
Certification: Yes
Model Number: IgM capture Aogarose HP

Payment & Shipping Terms:

Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 5ml, 25ml, 100ml, 200ml, 500ml, 1L etc.
Delivery Time: 10 working days
Payment Terms: T/T, L/C
Supply Ability: No limitation
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Detailed Product Description
Matrix: 6% Highly Cross-linked Agarose Average Particle Size: ~ 34μm
Ligand: 2-mercapto Pyridine Ligand Density: 2 Mg/ml Media
Binding Capacity: 5 Mg Human IgM /ml Media PH Stability (working): 3 ~ 11
Flow Rate/pressure: Max 150 Cm/h,max 3bar(0.3MPa) PH Stability (CIP): 2 ~ 13

 Affinity Chromatography Resin Human & Monoclonal IgM capture Aogarose HP

 

【CHARACTERISTICS】

 

The medium is prepared by coupling 2- mercaptopyridine to Bestaresin Diamond / HF / HP matrix .

Interactions among ligand and IgM is achieved by electron acceptance and donation effect-mixed hydrophilic and hydrophobic mode and mixed interaction.
 
  • Fast and efficient purification of monoclonal IgM.
  • High purification and recovery rate, excellent activity preservation.
 
【SPECIFICATION】
 
Matrix: 6% highly cross-linked agarose;
Average Particle size: ~34μm;
Ligand: 2-mercapto pyridine;
Ligand density: 2 mg/ml media;
Binding Capacity: 5mg human IgG/mL medium;
PH stability: 3 ~ 11 (working); 2~13 (CIP);
Flow rate/pressure: max 150 cm/h,max 3bar(0.3MPa);
Storage condition: 4 to 8°C, 20% Ethanol.
 
【APPLICATION CASE】

 

Chromatographic Column: RTU Fast 5ml IgM-HP x2

Sample: Mice ascites(contain IgG antibody) centrifuge12000rpm 20min, collect supernatent, filtrate with 0.45μm membrane, add(NH4)2SO4 to reach a final concentration of 0.8mol/L(NH4)2SO4,adjust pH for sampling.
Binding Buffer:0.8M(NH4)2SO4 20mM PB,pH 7.5
Elution Buffer:20mM PB, pH 7.5
Flow Rate:1ml/min

 

 

Affinity Chromatography media IgM capture Aogarose ( Sepharose ) HP

 
 
 

Contact Details
YELI BIOTECH (SHANGHAI) CO., LTD

Contact Person: emily

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