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Protein A Best Aogarose ( Sepharose ) Affinity Chromatography media

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Protein A Best Aogarose ( Sepharose ) Affinity Chromatography media

China Protein A Best Aogarose ( Sepharose ) Affinity Chromatography media supplier

Large Image :  Protein A Best Aogarose ( Sepharose ) Affinity Chromatography media

Product Details:

Place of Origin: China
Brand Name: AOGMA
Certification: Yes
Model Number: Protein A Best Aogarose

Payment & Shipping Terms:

Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 5ml, 25ml, 100ml, 200ml, 500ml, 1L etc.
Delivery Time: 10 working days
Payment Terms: T/T, L/C
Supply Ability: No limitation
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Detailed Product Description
Matrix: Highly Cross-linked Rigid Agarose Average Particle Size: ~ 75μm
Ligand: Recombinant Protein A-derived (E. Coli); Ligand Coupling Method: Epoxy;
Dynamic Binding Capacity: >28mg Human IgG/mL Medium (250cm/h, Diameter 10cm); PH Stability (working): 3 ~ 10
Flow Rate/pressure: ~ 500 Cm/h; Pressure<2bar; Bed Height 20cm; Diameter 5cm. PH Stability (CIP): 2 ~ 12

 Affinity Chromatography Resin Recombinant Protein A Best Aogarose

 

【CHARACTERISTICS】

  Protein A is a cell wall protein from Staphylococcus aureus.The Fc fragment can be specifically combined with mammalian IgG, and the gene of PROTEIN A is cloned by genetic engineering method. It is fermented and expressed by Escherichia coli, and the PROTEIN A is seperated and purified. No animal-derived components during fermentation and purification

  Protein A Best Aogarose is an affinity media developed by protein A crosslinked to an optimized rigid matrix Best Aogarose with epoxy chemistry. Highly cross-linked agarose matrix enables high loading capacity at low back pressure. The recombinant protein A ligand is designed for purification of monoclonal and polyclonal antibodies. 

 

  Advantages:

  • High flow rates at low back pressure with strong rigid matrix.
  • High dynamic loading capacity, cost-effective;
  • Easy Linear amplification, simple scale-up;
  • Extensive IgG binding capability.
 
【SPECIFICATION】
Matrix: Highly cross-linked rigid Agarose;
Average Particle size: ~75μm;
Ligand: Recombinant Protein A-derived (E. coli);
Coupling chemistry: Epoxy;
Dynamic Binding Capacity: > 28mg human IgG/mL medium (250cm/h, diameter 10cm);
PH stability: 3 ~10 (working); 2~12 (CIP);
Flow rate/pressure: Recommended velocity of 500 cm/h; Pressure<2bar; bed height 20cm; Diameter 5cm.
Storage condition: 4 to 8°C, 20% Ethanol.
 
【APPLICATION CASE】
 
Chromatographic Column: Protein A Best Aogarose,  RTU Screen
Sample: Human IgG in HCCF
Binding Buffer: PBS (20mMPB, 150mM NaCl, pH 7.4)
Elution Buffer: 100mM Gly, pH 2.7
Note: Eluted sample must be neutralized immediately with 1M Tris (pH9.0) buffer.
 
 
Protein A Best Aogarose ( Sepharose ) Affinity Chromatography media
 
 
 
 
 

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