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GST Aogarose ( Sepharose ) 4FF Gst Tagged Protein A affinity Chromatography media

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GST Aogarose ( Sepharose ) 4FF Gst Tagged Protein A affinity Chromatography media

China GST Aogarose ( Sepharose ) 4FF Gst Tagged Protein A affinity Chromatography media supplier

Large Image :  GST Aogarose ( Sepharose ) 4FF Gst Tagged Protein A affinity Chromatography media

Product Details:

Place of Origin: China
Brand Name: AOGMA
Certification: Yes
Model Number: GST Aogarose 4FF

Payment & Shipping Terms:

Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 10ml, 50ml, 100ml, 500ml, 1L
Delivery Time: 10 working days
Payment Terms: T/T, L/C
Supply Ability: No limitation
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Detailed Product Description
Matrix: 4% Agarose Average Particle Size: ~ 90μm
Ligand: Glutathione Ligand Coupling Method: Epoxy Activation
Binding Capacity: ~10mg Recombinant GST Protein/ml Medium PH Stability (working): 3 ~ 12
PH Stability (Cleaning-in-Place): 2 ~ 14 Flow Rate/Pressure: 50 ~300cm/h Recommended, Max 3bar

Affinity Chromatography Resin GST Tag Protein GST Aogarose 4FF

 

GST Aogarose 4FF is coupled to 4% agarose matrix by a 10 carbon linker, which gives the media a stronger binding capacity to GST-tagged proteins, the high binding capacity and excellent flow properties makes the media suitable for industrial production.

 

  It is suitable for recombinant fusion protein containing glutathione S- transferase at the carboxy terminus

 

 

SPECIFICATION

 

Matrix: Agarose, 4%, spherical
Average particle size: ~90μm
Ligand: Glutathione
Binding Capacity: ~10mg Recombinant GST protein/ml medium
Ligand Coupling Method Epoxy activation
PH working range: 3 ~ 12
PH stability (CIP): 2 ~ 14
Recommended flow rate: 50 ~300cm/h recommended, max 3bar
Storage: 4 ~ 30°C, 20% Ethanol.
Chemical Stability: Stable in commonly used aqueous buffers:0.1M NaOH, 70%Ethanol, 6M Guanidine Hydrochloride (1hour at room temerature),1M acetic acid

 

[APPLICATION CASE]

 

Chromatographic Column : RTU Fast 5 ml

Sample : Recombinant GST tag protein

Binding Buffer : 20mM PB, 150mM NaCl pH 7.4

Elution Buffer : Reducibility Glutathione 50mM Tris pH 8.0

 

GST Aogarose ( Sepharose ) 4FF Gst Tagged Protein A affinity Chromatography media

Contact Details
YELI BIOTECH (SHANGHAI) CO., LTD

Contact Person: emily

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