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Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands.

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Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands.

China Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands. supplier

Large Image :  Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands.

Product Details:

Place of Origin: China
Brand Name: AOGMA
Certification: Yes
Model Number: Phenyl Aogarose HP

Payment & Shipping Terms:

Minimum Order Quantity: Negotiable
Price: Negotiable
Packaging Details: 25ml, 200ml, 500ml or according to cusotmers' requirements.
Delivery Time: 5 ~ 10 working days after payment
Payment Terms: T/T, L/C
Supply Ability: No limitation
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Detailed Product Description
Matrix: Cross-linked Agarose, Spherical Average Particle Size: ~ 34μm
Ligand Concentration: 25µmol /ml Resin PH Working Range: 3 ~ 13
PH Stability (CIP): 2 ~ 14 Storage: 20% Ethanol
Ligand: Phenyl Product Name: Phenyl Aogarose HP

Hydrophobic Interaction Chromatography Resin aromatic hydrophobic Phenyl Aogarose HP

 

 Phenyl Aogarose HP is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

  • Aromatic HIC resin designed for intermediate purification and polishing steps
  • Selectivity similar to Phenyl Aogarose 6 FF (low sub) but higher resolution due to the smaller 34 µm bead size
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding
  • Suitable for fine separation of proteins containing aromatic ligands.

 

Average Particle Sizes:  ~ 34µm
Matrix Cross-linked agarose, spherical
PH working range: 3 ~ 13
PH stability (CIP): 2 ~ 14
Ligand Concentration 25µmol butyl/ml resin
Chemical Stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 1 M Acetic acid, 30% Acetonitrile, 70% Ethanol, 30% Isopropanol, 6 M guanidine-hydrochloride, 8 M Urea, , 3 M ammoniumsulfate, ionic detergents, non-ionic detergents, polar organic solvents, 2% sodium dodectyl sulfate
Storage: 4 to 30°C, 20% Ethanol

 

[Application Case]

 

(Phenyl Aogarose HP purify the yeast expression recombinant protein)

Medium : Phenyl Aogarose HP ; Column : CK50/30

Sample : 200ml some recombinant protein after capture

Equilibrium buffer : 20mM PB 1M NaCl pH 7.0

Elution buffer: 20mM PB pH 7.0

 

 

Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands.

1. Original sample;  2-9. Flow through;  10. Elution

 

 

Phenyl Aogarose ( Sepharose ) HP , for proteins containing aromatic ligands.

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YELI BIOTECH (SHANGHAI) CO., LTD

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